Mycosystema 5: 165-170, 1992
NEOHAPLOIDIZATION AND NEOHAPLONT MATING AS A MEANS OF IDENTIFICATION OF PLEUROTUS CULTURES
RONALD H. PETERSEN
Department of Botany, University of Tennessee Knoxville, Tennessee 37996 USA
ABSTRACT: Neohaploidization of dikaryon cultures of Pleurotus was performed usint oxgall agar techniques. Neohaplont isolates were monokaryotic and lacked clamp connections. These neohaplont isolates were mated with single- spore tester strains of formerly identified Pleurotus collections, and neohaplonts from each parent dikaryon were found to be compatible with tester strains of only one species. Such a process appears valuable as a means of identifying dikaryon Pleurotus cultures, and may be as useful for other fungal groups.