Mycosystema 5: 89-98, 1992


DNA FINGERPRINTING AND PATHOTYPE CHARACTERIZATION OF PYRICULARIA ORYZAE ISOLATES

ZHU HENG, JIANG WAN-RU, WANG JIU-LIN, LEI CAI-LIN, LING ZHONG-ZHUAN and ZHU LI-HUANG
Institute of Genetics, Academia Sinica, Beijing Institute of Crop Breeding and Cultivation, CAAS, Beijing


ABSTRACT: Based on an analysis of a genome library of the rice blast fungus, Pyricularia oryzae, a set of clones containing repeated sequences of P. oryzae was identified. Of them, POR6 and POR7 were proved highly polymorphic and random distributed in P. oryzae genomes. When POR6 was used as probe in DNA fingerprinting to investigate pathotypes of rice blast fungus races, distinctive genome-specific profiles were observed. We have analyzed the relationship between the pathotypes and ifngerprints of nine blast fungus isolates from Japan and China. The result indicated that the percentage of similar hybridization bands (Sxy) in POR6-DNA fingerprints of any two isolates are positively related to the number of rice cultivars which can be infected by these two isolates. POR6 may become a useful probe in study of diversity and phylogenetic relationship among races of rice blast fungus.