Mycosystema 1: 61-71, 1988


IDENTIFICATION OF RHIZOPUS BY ELECTROPHORESIS OF PROTEINS AND ESTERASES

HUANG HE and YU MING-QIU
Systematic Mycologh & Lichenology Laboratory, Institute of Microbiology, Academia Sinica, Beijing


ABSTRACT: An electrophoretic study of the buffer soluble mycelial protein and esterases of 24 isolates of 10 species of varieties of Rhizopus was carried out to obtain further information on the taxonomic relationships in the genus. Protein profiles were reproducible when the entire procedure of culturing, extraction and electrophoresis was repeated at different times under strictly controlled conditions with the same isolate. Under identical conditions, each species of Rhizopus produced a characteristic protein pattern. Intraspecific isolates generally gave very similar protein patterns and esterase zymograms, especially so with well-defined species like R. stolonifer and R. circinans. Protein patterns and zymograms of esterases are consequently thought to be able to play an effective supplenentary role in the cassification of Rhizopus.